Table 4. Top 50 most abundant T. pallidum proteins detected by MS analysis.
| TP number | UniProt Accession Number# | Protein Name | Molecular Weight (kDa) | Average NSAF value | cDNA/DNA signal ratio Smajs et al. [15] | GO Biological Process | Cellular location | COG category |
|---|---|---|---|---|---|---|---|---|
| TP0214 | R9UVZ6 | Uncharacterized protein | 7 | 19.6 | 1.6 | Unknown | no_hit | |
| TP0708 | R9UXB8 | Uncharacterized protein | 18 | 15.7 | 1.5 | Inner membrane | none | |
| TP0385 | O83400 | Uncharacterized protein | 14 | 13.6 | 1.2 | Unknown | none | |
| TP0951 | R9UVX4 | 50S ribosomal protein L34 | 6 | 13.1 | 1.8 | Translation | Ribosome | no_hit |
| TP0126a* | R9UTR8 | Uncharacterized protein | 23 | 13.1 | 1.1 | Unknown | none | |
| TP0474 | R9UUP5 | Probable transcriptional regulatory protein | 26 | 13.1 | 3.3 | Regulation of transcription, DNA-templated | Cytoplasm | K |
| TP0061 | R9UTJ4 | 30S ribosomal protein S18 | 12 | 12.5 | 1.2 | Translation | Ribosome | none |
| TP0048 | O83087 | Cell shape determination protein CcmA | 15 | 12.1 | 1.1 | Unknown | none | |
| TP0657 | R9UWD4 | Carbon storage regulator homolog | 8 | 12.0 | 0.9 | mRNA catabolic process; regulation of carbohydrate metabolic process | Unknown | none |
| TP0409a | R9UT20 | Preprotein translocase subunit YajC | 14 | 11.4 | 1.6 | Inner membrane | none | |
| TP0718 | R9UXC7 | IIISP family Type III (Virulence-related) secretory pathway protein/Flagellar biosynthesis protein FliP | 31 | 11.2 | 0.8 | Bacterial-type flagellum organization; protein secretion | Flagellum/Inner membrane | N |
| TP0808 | R9UWS1 | Acyl carrier protein | 9 | 10.1 | 2.8 | Fatty acid biosynthesis; Fatty acid metabolism | Cytoplasm | none |
| TP0558 | O83569 | Nickel/cobalt efflux system | 32 | 9.8 | 0.9 | Inner membrane | none | |
| TP0198 | R9USD7 | 30S ribosomal protein S17 | 10 | 9.8 | 1.5 | Translation | Ribosome | none |
| TP0849 | R9UU63 | 50S ribosomal protein L35 | 8 | 9.7 | 7.1 | Translation | Ribosome | no_hit |
| TP0490 | O83503 | Uncharacterized protein | 7 | 9.6 | 0.7 | Unknown | no_hit | |
| TP0649 | O83655 | Hemolysin (TlyC) | 30 | 9.5 | 0.6 | Inner membrane | P | |
| TP0213 | R9USF3 | 50S ribosomal protein L17 | 19 | 9.4 | 2.2 | Translation | Ribosome | J |
| TP0072 | O83111 | Glutaredoxin-related protein | 10 | 9.4 | 7.6 | Cell redox homeostasis | Unknown | none |
| TP0509 | O83522 | Alkyl hydroperoxide reductase (AhpC)/Peroxiredoxin | 21 | 9.3 | 15.5 | Unknown | O | |
| TP0919 | R9UVS2 | Thioredoxin | 11 | 9.2 | 8.1 | Cell redox homeostasis; glycerol ether metabolic process | Unknown | none |
| TP0362 | R9USW9 | 50S ribosomal protein L28 | 8 | 8.9 | 5.4 | Translation | Ribosome | none |
| TP0368 | R9UVP4 | Uncharacterized protein | 12 | 8.7 | 0.8 | Inner membrane | none | |
| TP0119 | O83156 | Amino acid ABC transporter, permease protein (YaeE) | 23 | 8.7 | 0.7 | Transport | Inner membrane | none |
| TP0195 | R9USH3 | 30S ribosomal protein S3 | 28 | 8.5 | 1.9 | Translation | Ribosome | J |
| TP0604 | R9UUZ6 | Ribosome-recycling factor | 21 | 8.4 | 1.6 | Protein biosynthesis; translation termination | Cytoplasm | J |
| TP0045 | R9UUU2 | Adenosine deaminase | 35 | 8.3 | 0.4 | Hydrolase activity; metal ion binding; deaminase activity; nucleotide metabolic process | Unknown | F |
| TP0700 | R9UV74 | Uncharacterized protein | 14 | 8.0 | 3.7 | Unknown | none | |
| TP0315 | R9USP4 | Uncharacterized protein | 23 | 8.0 | 0.9 | Unknown | none | |
| TP0922 | O83892* | Uncharacterized protein | 33 | 7.8 | 1.6 | Unknown | none | |
| TP0887 | R9UVP5 | 30S ribosomal protein S15 | 10 | 7.8 | 4.3 | Translation | Ribosome | none |
| TP0707 | R9UTN7 | Putative membrane protein | 17 | 7.6 | 0.6 | Inner membrane | none | |
| TP0202 | R9UTZ5 | 30S ribosomal protein S14 type Z | 7 | 7.6 | 1.8 | Translation | Ribosome | no_hit |
| TP0433 | R9UT05 | Acidic repeat protein | 66 | 7.6 | 1.4 | Unknown | none | |
| TP0663 | R9UV45 | Putative flagellar filament outer layer protein FlaA; Tromp-2 | 27 | 7.5 | 13.9 | Bacterial-type flagellum-dependent cell motility | Flagellum | none |
| TP0076 | R9UUX9 | Sugar ABC superfamily ATP binding cassette transporter, membrane protein | 30 | 7.4 | 0.4 | Transport | Inner membrane | G |
| TP1029 | R9UY97 | Uncharacterized protein | 25 | 7.4 | 0.9 | Unknown | none | |
| TP0249 | R9UVD9 | Flagellar filament outer layer protein (FlaA1) | 39 | 7.4 | 16.1 | Flagellum | N | |
| TP1012 | O83976 | RNA polymerase sigma factor | 35 | 7.4 | 0.5 | DNA-templated transcription; initiation | Polymerase | K |
| TP0206a | R9UU00 | 50S ribosomal protein L30 | 7 | 7.4 | 2.0 | Translation | Ribosome | no_hit |
| TP0194 | R9UVW4 | 50S ribosomal protein L22 | 14 | 7.3 | 1.8 | Translation | Ribosome | J |
| TP0234 | R9UU20 | 50S ribosomal protein L33 | 7 | 7.2 | 4.2 | Translation | Ribosome | no_hit |
| TP0007 | R9URZ2 | Uncharacterized protein | 35 | 7.1 | 1.8 | Unknown | none | |
| TP0412 | O83427 | Uncharacterized protein | 12 | 7.1 | 1.2 | Unknown | S | |
| TP0792 | R9UU06 | Flagellin/ Flagellar filament 33 kDa core protein; Class B | 31 | 7.0 | 13.8 | Bacterial-type flagellum-dependent cell motility | Flagellum | N |
| TP0654 | O83660 | Spermidine/putrescine ABC superfamily ATP binding cassette transporter, membrane protein | 30 | 6.9 | 1.0 | Transport | Inner membrane | none |
| TP0563 | O83574 | Uncharacterized protein | 16 | 6.9 | 0.8 | Unknown | O | |
| TP0248 | O83276* | Uncharacterized lipoprotein TP_0248 | 15 | 6.8 | 1.5 | Lipoprotein- unknown membrane localization | S | |
| TP0403 | O83418 | Flagellar biosynthesis protein FliJ | 18 | 6.8 | 0.8 | Bacterial-type flagellum-dependent cell motility; chemotaxis | Flagellum | none |
| TP0842 | R9UU24 | Methionine aminopeptidase | 27 | 6.8 | 0.8 | Protein initiator methionine removal | Unknown | J |
Legend:
#: UniProt Proteome ID: UP000014259; NA: not available; NSAF normalized spectral abundance factor; no_hit: proteins with no UBLAST hit to any eggNOG sequence; none: over 70% of hits to a member that is not assigned an eggNOG code.
*: the C-terminal 49 amino acids of TP0126a correspond to the N-terminal 49 amino acids of TP0126 due to a genome insertion [29]