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. 2016 Sep 8;12(9):e1005869. doi: 10.1371/journal.ppat.1005869

Fig 7. 19F-K+-uptake mutants are not sensitive to daptomycin or valinomycin.

Fig 7

A) 19F has three K+-uptake-systems; system-1 (SP0480, SP0479), system-2 (SP0078, SP0079), and system-3, which is strain-specific for 19F (SPT1006). B-D) K+-uptake-systems-1, 2 and 3 are not important for growth in the presence of daptomycin. E-H) Double mutants of the three systems also have no significant effect on growth in the presence of daptomycin or valinomycin (only growth in the presence of valinomycin for double mutant Trk1-SPT1006 is shown). Note that higher concentrations of daptomycin were used compared to Fig 5, to illustrate that the absence of sensitivity is independent of daptomycin concentration. H) Red and blue lines depict the wild type and mutant strain respectively without daptomycin, while yellow and green lines depict the wild type and mutant strain respectively with daptomycin.