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. 2016 Aug 23;57(10):4287–4294. doi: 10.1167/iovs.16-19937

Figure 4.

Figure 4

Drugs alter IHMGEC signaling. Cells were cultured for 6 days in DMEM/F12 supplemented with 10% FBS and 10 ng/mL EGF, serum starved overnight (1% FBS), and treated with drugs or vehicles for 15 minutes. Cell lysates were transferred to PVDF and incubated with antibodies specific for phospho-AKT or β-actin. Insulin-like growth factor (IGF-1, 10 nM) is a positive control. Band intensity was normalized to actin and analyzed using ImageJ. By analysis of variance, significant differences exist between groups: P < 0.0001. Post hoc analysis using Dunnett's multiple comparisons test indicates that individual treatments significantly decreased (*P < 0.05; **P < 0.01; ***P < 0.001) or significantly increased (†P < 0.001) AKT phosphorylation compared to control.