Skip to main content
. 2016 Sep 8;14(9):e1002553. doi: 10.1371/journal.pbio.1002553

Fig 5. NANOS2 upregulation occurred in Smad4-null germ cells in the absence of RA signaling.

Fig 5

(A) Scheme of strategy to trace the fate of Smad4-null germ cells. Deletion of Smad4 by Cre recombination induces the expression of GFP, indicating Smad4-null germ cells. (B) Representative image of sections from Smad4 (Stella) ovaries after DMSO or AGN193109 treatment stained for NANOS2 and GFP. White arrows indicate GFP-positive cells. Ovaries were cultured from E12.5 for 3 d with indicated drugs after injection of tamoxifen at E9.5 and E10.5. Scale bars: 50 μm.