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. 2016 Sep 8;14(9):e1002553. doi: 10.1371/journal.pbio.1002553

Fig 6. Deletion of Smad4 and Stra8 induces XX germ cells to adopt a male fate.

Fig 6

(A–C) Representative image of E15.5 Smad4-KO (Smad4 (Stella), Stra8+/-), Stra8-KO (Smad4f/f, Stra8-/-), and DKO ovary sections stained for SYCP3/NANOS2 (A), SYCP3/DNMT3L (B), and E-CADHERIN/NANOS2/TRA98 (C). High magnification images are shown in different channels. Numbers represent the percentage (%) of NANOS2-positive cells among SYCP3 -positive cells. Yellow arrowheads indicate NANOS2/E-CADHERIN/TRA98 triple-positive cells, and white arrowheads indicate TRA98-single positive cells. (D,E) Representative images of DKO ovary sections stained for 5-mC/DNMT3L and PLZF/E-CADHERIN. Tamoxifen was injected at E9.5 and E10.5. Gonads were cultured for 4 d from E14.5. White arrows in D and E indicate 5-mC-positive and PLZF-positive germ cells, respectively. Scale bars: 50 μm.