Figure 6.

Leukocytes isolated from diabetic mice (diabetic 2 or 8 months) caused more cytotoxicity to retinal endothelial cells than did leukocytes from age-matched nondiabetic mice. Leukocytes isolated from mice deficient in opsin or containing the P23H mutation caused cytotoxicity to endothelial cells while photoreceptors were present and degenerating (age 2 and 4 months), but not after the photoreceptors were absent (10 months). Circles: C57Bl/6J; triangles: opsin^−/−; squares: P23H. Nondiabetic, solid symbols; diabetic, hollow symbols. Symbols have been offset slightly to allow visualization of data. *P < 0.05 or ***P < 0.001 compared to N C57Bl/6J controls; †P < 0.05 or †††P < 0.001 compared to D C57Bl/6J controls. n = 3 to 5 in all groups. N, nondiabetic; D, diabetic.