Fig 1. Ability of recombinant Stx1B to bind to Gb₃ receptor in vitro.

(A) Binding of serially diluted Stx1B to Gb₃ as determined by ELISA using THE^™ His tag antibody. A₄₅₀: Absorbance at 450 nm. Error bars denote standard deviations. (B) Internalization of FITC-labelled Stx1B into HeLa cells. Golgi apparatus was detected with mouse anti-human Golgin-97 primary antibody and Alexa Fluor 555-conjugated donkey anti-mouse secondary antibody (red). DAPI staining was used to label nuclei (blue). Bars = 10 μm.