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. 2016 Sep 8;14(9):e2000197. doi: 10.1371/journal.pbio.2000197

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© 2016 Santos et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 7 — A. Table summarizing genotypes, heart size, body size and incidence of ASDs in different crosses. B. Rescued Nipbl^FLEX/+;cTnt-Cre embryos (n = 14) resembled their Nipbl^FLEX/+ littermates (n = 22) in body size and were smaller than control littermates (cTnt-Cre n = 18, wildtype n = 31). C. Similar results were observed in rescued Nipbl^FLEX/+;Sox17-2A-iCre embryos (n = 22; Sox17-2A-iCre n = 16; Nipbl^FLEX/+ n = 18; wildtype n = 25). D. Nipbl^Flox/+;cTnt-Cre (n = 15) were similar in overall body size to littermate controls (Nipbl^Flox/+ n = 14, cTnt-Cre n = 30, wildtype n = 21). E. Similar results were observed in Nipbl^Flox/+;Sox17-2A-iCre (n = 13) when compared to littermate controls (Sox17-2A-iCre, n = 20; Nipbl^Flox/+ n = 10; wildtype n = 19). Note that individual weights for each cross in B–E were normalized to the mean weight of cTnt-Cre controls (B, D), or Sox17-2A-iCre controls (C, E); black bars indicate normalized mean weight for each genotype. F. Ventricular volume analyses (graphed as box plots) show that the overall heart size of rescued Nipbl^FLEX/+;cTnt-Cre embryos (n = 7) were similar in size to Nipbl^FLEX/+ heart size (n = 9) (Mann-Whitney U, p > 0.05). Control hearts (cTnt-Cre, n = 6) were significantly larger than the hearts of their Nipbl^FLEX/+ and Nipbl^FLEX/+;cTnt-Cre littermates (asterisks: Mann-Whitney U, p < 0.05). G. Rescued Nipbl^FLEX/+;Sox17-2A-iCre embryo hearts (n = 5) were also similar in size to Nipbl^FLEX/+ littermate heart size (n = 6) (Mann-Whitney U, p > 0.05) and significantly smaller than control hearts (Sox17-2A-iCre, n = 5) (asterisk: Mann-Whitney U, p < 0.05). H. Ventricular volume analysis show that the ventricle size of Nipbl^Flox/+;cTnt-Cre embryos (n = 9), which display a high frequency of heart defects, were similar in size to control hearts (cTnt-Cre, n = 9) (Mann-Whitney U, p > 0.05). I. Nipbl^Flox/+;Sox17-2A-iCre mutant hearts (n = 5), which also display a high frequency of heart defects, were also similar in size to control hearts (Sox17-2A-iCre, n = 5) (Mann-Whitney U, p > 0.05).