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. Author manuscript; available in PMC: 2017 Sep 2.
Published in final edited form as: Circ Res. 2016 Jul 18;119(6):e91–e103. doi: 10.1161/CIRCRESAHA.116.308955

Figure 4. Cholesterol loading increases while cholesterol unloading decreases platelet and PKC activity.

Figure 4

(a) Platelets from the chow-fed recipients were loaded with or without cholesterol and then stimulated with AYPGKF (100μM). Surface P-selectin levels were shown (n=4). (b) Platelets from the chow-fed recipients were loaded with or without cholesterol and then stimulated with or without AYPGKF (50μM). PKC activity was estimated (n=3). AYPGKF-induced surface P-selectin exposure (c) or aggregation (d) of platelets from the WTD-fed recipients, with or without rHDL (50 μg/ml) treatment. (e, f) similar as in (c) or (d) except that cyclodextrin (CD) (3mM) replaced HDL for the treatment (n=4). (g) PKC activity in platelets from WTD-fed recipients was estimated, with and without AYPGKF (100μM) and HDL (50μg/ml) treatment (n=3).

For 3c and 3e, * p<0.05 between indicated groups; # p<0.05 between control (WTD) and CD or HDL treatment groups.