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. 2016 Sep 8;11(9):e0161229. doi: 10.1371/journal.pone.0161229

Fig 5. Expression of pluripotency markers in hPSCs cultured for 40 passages using the defined, xeno-free EOD hPSC medium.

Fig 5

Three hPSC lines (two replicates per line) were evaluated for their ability to sustain the expression of markers of pluripotency at passage 10, passage 25 and passage 40. Each hPSC line was independently analyzed by flow cytometry for the expression of SSEA-4, TRA-1-60 and TRA-1-81. Results within each passage were combined and the mean value of marker expression determined. Bars represent mean percentage values. Error bars represent the standard deviation of the mean for each passage.