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Purification of hydrazine dehydrogenase from K. stuttgartiensis
| Step | Protein | Volume | Total protein | Specific activity | Total activity | Yield | Purification |
|---|---|---|---|---|---|---|---|
| mg ml−1 | ml | mg | μmol min−1 mg protein−1 | μmol min−1 | % | -fold | |
| Cell extract | 53 | 10 | 530 | 0.55 | 292 | 100 | 1 |
| Q Sepharose | 11 | 6 | 66 | 1.1 | 73 | 25 | 2 |
| Hydroxyapatite | 0.6 | 12 | 7.2 | 8.4 | 60 | 24 | 15 |
