Reaction products formed from 17α-hydroxyprogesterone
and 17α-hydroxypregnenolone in P450 17A1 reactions
supported by various factors. Retention times
(tR) and integration units are
indicated on the chromatograms. A–C,
17α-hydroxyprogesterone; D–F,
17α-hydroxypregnenolone. A and
D, NADPH-P450 reductase, cytochrome
b5, and NADPH;
B and E,
H2O2 (10 mm) (with cytochrome
b5); C and
F, iodosylbenzene (PhI=O,
300 μm) (with cytochrome
b5). In these studies the
Δ5 products (formed from
17α-hydroxypregnenolone) were oxidized to
Δ4 products to facilitate LC-UV
analysis.