Identification of 16-hydroxy steroids as reaction products
formed from DHEA and androstenedione.
A, authentic steroid standards:
16α-hydroxyandrostenedione, algestone
(16α,17α-dihydroxyprogesterone), androstenedione,
and 17α-hydroxyprogesterone; B, 10-min
DHEA incubation (with products treated with cholesterol
oxidase); C, 10-min androstenedione incubation;
D, mass spectrum of peak identified as
16-hydroxyandrostenedione (formed from androstenedione);
E, MS/MS analysis of m/z
303.2 peak of D.