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. 2016 Jun 23;291(33):17143–17164. doi: 10.1074/jbc.M116.732966

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 11. — Identification of 16-hydroxy steroids as reaction products formed from DHEA and androstenedione. A, authentic steroid standards: 16α-hydroxyandrostenedione, algestone (16α,17α-dihydroxyprogesterone), androstenedione, and 17α-hydroxyprogesterone; B, 10-min DHEA incubation (with products treated with cholesterol oxidase); C, 10-min androstenedione incubation; D, mass spectrum of peak identified as 16-hydroxyandrostenedione (formed from androstenedione); E, MS/MS analysis of m/z 303.2 peak of D.