TABLE 2.
Plasmids used in this study
| Plasmid | Description | Source or reference |
|---|---|---|
| pcDNA3 | Invitrogen | |
| pCISII-rAANAT | rAANAT in pCISII | Ref. 98 |
| pRS313Cup1 | pRS313 containing the PCUP1 promoter | Varshavsky laboratory collection |
| pRS416Gal1 | pRS416 containing the PGAL1 promoter | Varshavsky laboratory collection |
| pBW105 | MLSrAANAT3f in pRS416 with PGal1 | This study |
| pBW106 | (M)SIrAANAT3f in pRS416 with PGal1 | This study |
| pBW107 | (M)SMLSrAANAT3f in pRS416 with PGal1 | This study |
| pBW135 | MLSrAANAT3ha in pRS313 with PCup1 | This study |
| pBW173 | pcDNA3 (PCMVt1) | This study |
| pBW206 | MLSrAANAT3f in pcDNA3 (PCMVt1) | This study |
| pBW209 | (M)PLSrAANAT3f in pRS416 with PGal1 | This study |
| pBW211 | (M)SMLSrAANAT3f in pcDNA3(PCMVt1) | This study |
| pBW212 | (M)SIrAANAT3f in pcDNA3(PCMVt1) | This study |
| pBW213 | (M)PLSrAANAT3f in pcDNA3(PCMVt1) | This study |
| pBW394 | MLSrAANAT3haK8R in pRS416 with PGal1 | This study |
| pBW395 | (M)SThAANAT3f in pRS416 with PGal1 | This study |
| pBW419 | (M)PThAANAT3f in pRS416 with PGal1 | This study |
| pBW466 | (M)SThAANAT3f in pcDNA3(PCMVt1) | This study |
| pBW467 | (M)PThAANAT3f in pcDNA3(PCMVt1) | This study |
| pBW477 | MLSrAANAT3ha in pcDNA3 (PCMVt1) | This study |
| pBW478 | MLSrAANAT3haKzero in pcDNA3 (PCMVt1) | This study |
| pBW481 | (M)SThAANAT3f in pcDNA3(PCMVt1) | This study |
| pBW482 | (M)SThAANAT3haKzero in pcDNA3(PCMVt1) | This study |