TABLE 1.
Summary of the values for the 3-s isochronal activation V0.5 and time constant of activation at 20 mV for S4S5 alanine mutants
Data are presented as mean ± S.E. (n), and n denotes number of individual recordings. Asterisk indicates p < 0.05 versus WT using one-way ANOVA with Bonferroni post-test.
| Mutant | Activation V0.5 (mV) (n) | Time constant of activation at +20 mV (ms) (n) |
|---|---|---|
| WT | −23.9 ± 1.3 (5) | 102.5 ± 6.1 (8) |
| D540A | −23.1 ± 1.7 (5) | 55.2 ± 2.2* (5) |
| R541A | −33.2 ± 1.3* (4) | 87.0 ± 3.6 (5) |
| Y542A | −7.2 ± 1.5* (4) | 128.0 ± 11.0 (6) |
| S543A | −45.5 ± 1.2* (4) | 64.2 ± 1.6* (7) |
| E544A | −25.7 ± 0.7 (3) | 97.8 ± 2.6 (6) |
| Y545A | −36.0 ± 1.2* (5) | 97.3 ± 4.5 (7) |
| G546A | −65.2 ± 2.2* (3) | 63.0 ± 2.8* (5) |
| A547V | −13.0 ± 1.8* (3) | 157.2 ± 6.0* (6) |
| A548V | −55.3 ± 0.8* (5) | 55.0 ± 5.1* (7) |
| V549A | −13.3 ± 1.1* (4) | 225.8 ± 15.0* (7) |