TABLE 2.
Summary of the values for the 3-s isochronal deactivation V0.5, time constants of deactivation at −70 and −120 mV for S4S5 alanine mutants
Data are presented as mean ± S.E., and n denotes number of individual recordings. Asterisk indicates p < 0.05 versus WT using one-way ANOVA with Bonferroni post-test.
| Mutant (n) | Deactivation V0.5 (mV) | Time constant of deactivation at −120 mV (ms) | Time constant of deactivation at −70 mV (ms) |
|---|---|---|---|
| WT (5) | −61.3 ± 0.5 | 27.8 ± 1.3 | 286.2 ± 32.4 |
| D540A (5) | −25.6 ± 2.0* | 23.8 ± 1.4 | 41.7 ± 1.4* |
| R541A (4) | −46.3 ± 0.8* | 20.4 ± 0.9 | 124.9 ± 7.5* |
| Y542A (5) | −1.6 ± 1.1* | 14.3 ± 0.5* | 29.7 ± 0.7* |
| S543A (4) | −78.6 ± 0.6* | 49.3 ± 3.6* | NA |
| E544A (3) | −40.3 ± 1.4* | 21.1 ± 1.8 | 113.7 ± 9.0 * |
| Y545A (5) | −46.4 ± 1.1* | 14.4 ± 1.1* | 110.4 ± 18.6* |
| G546A (4) | −82.0 ± 0.2* | 64.7 ± 2.2* | NA |
| A547V (3) | −56.5 ± 1.1 | 31.2 ± 2.8 | 217.3 ± 3.4 |
| A548V (5) | −64.8 ± 0.6 | 45.1 ± 1.7 * | 268 ± 21.8 |
| V549A (4) | −53.3 ± 1.1* | 28.4 ± 3.1 | 208.7 ± 15.9 |