TABLE 3.
Summary of the values for the ΔΔG0 (mean ± S.E.) and n denotes number of individual recordings
Asterisk means data are statistically different from their corresponding additive effect.
| Mutant (n) | ΔG0(exp) | ΔΔG0(Mut − WT) | ΔΔG0(Mut1 + Mut2) − (ΔΔG0(Mut1) + ΔΔG0 (Mut2)) |
|---|---|---|---|
| kJ·mol−1 | kJ·mol−1 | kJ·mol−1 | |
| WT(5) | −22.7 ± 0.6 | ||
| Y542A(5) | −0.3 ± 0.2 | 22.3 ± 0.2 | |
| V535A(3) | −20.8 ± 1.8 | 1.8 ± 1.8 | |
| R537A(4) | −20.1 ± 0.6 | 2.6 ± 0.6 | |
| I560A(3) | −22.1 ± 1.0 | 0.5 ± 1.0 | |
| Y542A/V535A(5) | −1.8 ± 0.1 | 20.8 ± 0.1 | −3.4 ± 0.9* |
| Y542A/R537A(3) | 0.3 ± 0.1 | 22.9 ± 0.1 | −2.0 ± 0.4* |
| Y542A/I560A(4) | −4.4 ± 1.1 | 18.2 ± 1.1 | −4.6 ± 0.8* |
| Y545A(5) | −19.5 ± 0.6 | 3.2 ± 0.6 | |
| I560A(3) | −22.1 ± 1.0 | 0.5 ± 1.0 | |
| S654A(4) | −23.7 ± 1.0 | −1.1 ± 1.0 | |
| N658A(4) | −17.2 ± 1.1 | 5.5 ± 1.1 | |
| Y545A/I560A(4) | −21.0 ± 1.2 | 1.7 ± 1.2 | −2.0 ± 0.9 |
| Y545A/S654A(3) | −20.1 ± 0.4 | 2.6 ± 0.4 | 0.5 ± 0.7 |
| Y545A/N658A(4) | −13.7 ± 1.2 | 8.9 ± 1.2 | 0.3 ± 1.0 |