Neuritin-induced Kv4.2 expression and IA densities require NFATc4.
A, effect of neuritin on Kv4.2 protein expression in CGNs isolated from wild type mice (C57BL/6), Nfatc2−/−, Nfatc4−/−, and the combined Nfatc2−/− and Nfatc4−/− DKO mice measured by Western blotting. B, induction of IA densities recording from C57BL/6 and Nfatc4−/− mouse CGNs after neuritin stimulation. C, induction of Kv4.2 mRNA level measured by quantitative RT-PCR from C57BL/6 and Nfatc4−/− mouse CGNs after neuritin stimulation. The data were obtained from three independent experiments. D, representative sample showing Western blotting-detected expression of NFATc4 protein in mouse cerebellum and PFC from C57BL/6 and Nfatc4−/− mice. *, p < 0.05; **, p < 0.01; and ***, p < 0.001 for two groups connected with a straight line by one-way ANOVA followed by Fisher's post hoc test.