FIGURE 3.

Neuritin induces dephosphorylation and nuclear accumulation of NFATc4. A, the levels of NFATc4 Ser¹⁶⁸ and Ser¹⁷⁰ phosphorylation (p-NFATc4) after neuritin treatment for 20 min in mice CGNs were examined by Western blotting. B and C, representative recording sample and statistical analysis showing effect of neuritin on nuclear accumulation of NFATc4 was examined by confocal microscopy in HeLa cells, which expressed IR activated by neuritin. The effects of CaN inhibitor CsA and IR inhibitor HNMPA are also shown. The percentage of nuclear NFATc4 in 100 cells was counted and presented. D, effect of neuritin on nuclear NFATc4 protein in mice CGNs by Western blotting analysis. E, effect of neuritin on cytoplasmic NFATc4 protein in mice CGNs by Western blotting analysis. *, p < 0.05; and ***, p < 0.001 for two groups connected with a straight line by one-way ANOVA followed by Fisher's post hoc test.