Effect of neuritin on the Kv4.2 gene promoter.
A, effect of neuritin on the Kv4.2 gene promoter (−2181 to +191 bp) was determined by luciferase reporter assays. The effect of deleting NFAT binding sites to −1513 bp (promoter Δ) is shown. B, recruitment of NFATc4 to the Kv4.2 gene promoter was examined by chromatin immunoprecipitations. Primers for PCR amplification (pairs 1, 2, and 3) are illustrated. ***, p < 0.001 for two groups connected with a straight line by one-way ANOVA followed by Fisher's post hoc test.