FIGURE 7.

Requirement of NFATc4 in neuritin-mediated formation of dendritic spines in PFC neurons. A and B, representative images and statistical analyses of the effect of neuritin on PFC neuron spine density in C57BL/6 and Nfatc4^−/−mice. Identification of neurons was performed using Golgi staining. C, effect of neuritin on spine length of PFC neurons in C57BL/6 or Nfatc4^−/−mice. Cumulative frequency distributions of spine length were analyzed using a one-way Kruskal-Wallis test followed by Kolmogorov-Smirnov test. The median and quartiles of the control group and neuritin-treated group were compared. D, Sholl analysis of the branch intersection numbers. E and F, effect of neuritin on first and second order dendrite length of PFC neurons in C57BL/6 or Nfatc4^−/− mice. ***, p < 0.001 compared with corresponding control by one-way ANOVA followed by Fisher's post hoc test.