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. 2016 Jun 17;291(33):17417–17426. doi: 10.1074/jbc.M116.727743

FIGURE 5.

FIGURE 5.

The heme transporter from C. elegans (CeHRG-4) also suppresses the respiratory phenotype in hem15Δ and hem1Δ cells; however, the mechanism of suppression could be different from that mediated by the Nce102 suppressor. A, growth test of hem1Δ and hem15Δ cells expressing the CeHRG-4 protein on a multicopy plasmid showing the rescue mediated by the heme transporter. B, β-galactosidase assay of hem1Δ cells bearing either the nce102 suppressor allele or expressing the CeHRG-4 plasmid that were grown in the presence of the indicated concentrations of hemin. C, growth test in the presence of the toxic heme analog gallium PPIX of WT yeast cells transformed with a plasmid bearing CeHRG-4 or cells possessing the Nce102 A125E mutation. D, CcO activities from purified mitochondria of hem15Δ cells bearing either the nce102 suppressor allele or expressing the CeHRG-4 plasmid. E, SDS-PAGE analysis of purified mitochondria showing the restoration of Cox2 subunit of CcO relative to hem15Δ nce102 suppressor allele or hem15Δ cells harboring the CeHRG-4 plasmid. Gly/Lac, glycerol/lactate; Raff, raffinose; GaPPIX, gallium PPIX.