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In vitro Alpha binding assay. A, saturation binding experiment of RdRp with truncated 3′-SL. The Alpha-binding signal is shown in fluorescence units (FU). B, competition assay with unlabeled RNA. RdRp and Bi-miniSL concentrations were kept constant at 200 and 10 nm, respectively. Mean and standard deviation values are derived from three independent experiments. We note that SLA did not show a binding signal in both saturation binding and competition assay.
