TABLE 2.
Primers used in real time quantitative PCR
| Gene name | Accession no. | Forward primer sequence (5′–3′) | Reverse primer sequence (5′–3′) |
|---|---|---|---|
| Actin | NM_007393.5 | GGCTGTATTCCCCTCCATCG | CCAGTTGGTAACAATGCCATGT |
| arginase-1 | NM_007482.3 | CTCCAAGCCAAAGTCCTTAGAG | AGGAGCTGTCATTAGGGACATC |
| IL-10 | NM_010548.2 | GCTCTTACTGACTGGCATGAG | CGCAGCTCTAGGAGCATGTG |
| CD206 | NM_008625.2 | CTCTGTTCAGCTATTGGACGC | CGGAATTTCTGGGATTCAGCTTC |
| caspase-6 | NM_009811.4 | GGAAGTGTTCGATCCAGCCG | GGAGGGTCAGGTGCCAAAAG |
| MMP-2 | NM_008610.3 | CAAGTTCCCCGGCGATGTC | TTCTGGTCAAGGTCACCTGTC |
| MMP-9 | NM_013599.4 | CTGGACAGCCAGACACTAAAG | CTCGCGGCAAGTCTTCAGAG |
| VEGF | NM_001025250.3 | GCACATAGAGAGAATGAGCTTCC | CTCCGCTCTGAACAAGGCT |
| TNF-α | NM_001278601.1 | CCCTCACACTCAGATCATCTTCT | GCTACGACGTGGGCTACAG |
| IL-1β | NM_008361.4 | GAAATGCCACCTTTTGACAGTG | TGGATGCTCTCATCAGGACAG |
| gnpnat1 | NM_019425.2 | ATGAAACCCGATGAAACTCCC | GCCTCAAAACCAAGCCTTCTC |
| ahnak | NM_001039959.2 | GGTCCCAAATTCAGGATGCCT | GGGCCTTCAAAATCCAGACCT |
| plec1 | NM_011117.2 | GGCTGAAGGTAGTTCCAATGG | GTGCCTCTGAGCCTTGATAAG |
| sptbn1 | NM_175836.2 | CCACCTTGCGAGAGTGTCC | GTCCCTTAGTGGGTTTAGGCA |
| ubr4 | NM_001160319.1 | GGGACGCCACCTTCTAACAG | TTCAGAGTGTTCGTCTCCAGC |
| vps13c | NM_177184.3 | GAAGCTAAAGTAAAAGCCCACGA | ACACATCAGAGGTGTTGACAATG |
| slc16a1 | NM_009196.4 | TGTTAGTCGGAGCCTTCATTTC | CACTGGTCGTTGCACTGAATA |
| map4 | NM_001205330.1 | CAGTCTTGTGGATGCGTTGAC | TTCCCGGTTTTCTCATCACCA |
| hmox1 | NM_010442.2 | AAGCCGAGAATGCTGAGTTCA | GCCGTGTAGATATGGTACAAGGA |
| soat1 | NM_009230.3 | GAAGGCTCACTCATTTGTCAGA | GTCTCGGTAAATAAGTGTAGGCG |