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. 2016 Jun 29;291(34):17496–17509. doi: 10.1074/jbc.M116.740308

FIGURE 4.

FIGURE 4.

Gadd45a activates MEKK4 in mouse skeletal muscle. A, one TA per mouse was transfected with 10 μg of empty pcDNA plasmid, and the contralateral TA in each mouse was transfected with 10 μg of Gadd45a-FLAG plasmid, as indicated. Seven days post-transfection, bilateral TA muscles were harvested for immunoblot analysis using the indicated antibodies. B, mouse TA muscles were transfected with 5 μg of empty pcDNA plasmid, 5 μg of MEKK4-FLAG plasmid, 20 μg of control RNAi plasmid, and/or 20 μg of MEKK4 RNAi plasmid, as indicated. Seven days post-transfection, TA muscles were harvested for immunoblot analysis using an anti-FLAG antibody. C, one TA per mouse was transfected with 10 μg of Gadd45a-FLAG plasmid plus 20 μg of control RNAi plasmid, and the contralateral TA in each mouse was transfected with 10 μg of Gadd45a-FLAG plasmid plus 20 μg of MEKK4 RNAi plasmid, as indicated. Seven days post-transfection, bilateral TA muscles were harvested for immunoblot analysis using the indicated antibodies.