FIGURE 2.

Specificity of the interaction between TH and HSC70. A, immunoblot analysis showing the expression of Hsc70, HSP70, GRP78, and HDP90 from MN9D cells under control conditions or after heat shock (HS). B, co-immunoprecipitation (IP) experiments were performed by incubating MN9D cell lysates with anti-TH antibody. SDS-PAGE and IB analysis using the designated antibodies demonstrated co-precipitation of Hsc70 but not HSP70, GRP78, or Hsp90. No bands were present in any of the immunoprecipitations performed using nonspecific IgGs.