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. 2016 Jun 30;291(34):17510–17522. doi: 10.1074/jbc.M116.728782

FIGURE 6.

FIGURE 6.

Hsc70 expression affects TH activity and intracellular DA concentrations in MN9D cells. A, representative IBs and densitometry analysis of MN9D cells, WT or stably transfected with scramble shRNA (Scr) or Hsc70 shRNA. Hsc70 expression was reduced in Hsc70 shRNA MN9D cells by ∼50%, whereas TH, Hsp70, and sodium/potassium ATPase expression was not significantly altered. Densitometry analysis of Hsc70 expression was normalized to arbitrary densitometry units of α-tubulin and expressed as a percentage of Hsc70 expression in MN9D WT cells. B, TH activity was examined in the PNS of these samples. The Hsc70 shRNA MN9D cells exhibited an ∼80% reduction in TH activity compared with WT and scramble shRNA stably transfected cells. C, intracellular DA levels present in the PNS of these samples were measured by HPLC. The DA levels present in the Hsc70 shRNA cells were reduced by ∼40% compared with WT and scramble shRNA MN9D cells. D, in contrast, transfection of MN9D cells with Hsc70-HA resulted in increased TH activity compared with MN9D WT cells (Control). *, p < 0.05.