FIGURE 10.

Smo localizes to the cilia in response to Hh ligand in the ΔML mutant of Ptch1. Ptch^−/− MEFs transiently transfected with Ptch1^FL (A) or ΔML (B) were serum-starved for 48 h prior to treatment with either control or N-Shh conditioned medium for 8 h. Cells were fixed in 4% paraformaldehyde and probed for acetylated tubulin (acTub, blue), Ptch1 (red), and Smo (green). Smo enters the cilia in response to Shh ligand with either Ptch1^FL or ΔML. C, straight Western blots were run using lysates taken from identical wells as the coverslips used for immunofluorescence staining. Staining and Western blots are representative of three independent experiments.