FIGURE 7.

Posttranslational processing occurs normally in mPtch1 mutants. Ptch1 deletion mutants exhibit correct secretory pathway trafficking in HEK 293 cells. Equal amounts of Ptch1^FL, ΔML, ΔC, ΔMLΔC, ΔMLΔC^X-A, ΔMLΔC^Xcm, and ΔMLΔC^P-A were transfected into HEK 293 cells. Cell lysates were taken and subjected to Endo H or PNGase F treatment for 1 h. All Ptch1 constructs tested, except ΔMLΔC^P-A, are partially resistant to Endo H cleavage, indicating correct posttranslational processing and secretory pathway trafficking.