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Vam7-6A affects PI3P binding by the PX domain. A, liposomes with PI3P were incubated with wild type Vam7, Vam7-6A, Vam7Y42A, or Vam7-7A. Protein binding was detected as described above and compared with Vam7-6A bound to PI3P. IB, immunoblot. B, Vam7-6A and PX were incubated with PI3P liposomes. The bar graph indicates average PI3P binding normalized to Vam7-6A. Error bars represent S.E. (n = 3). *, p < 0.05.
