P. aeruginosa ChpA domain architecture and protein constructs used in these studies. Predicted domains are represented by rectangles with Xpt domains (solid gray), combined dimerization/HATPase_c/CheW domains (vertical stripe), and receiver domains (diagonal stripe). A, domain architecture of full-length ChpA with conserved His (H), Thr (T), Ser (S), and Asp (D) labeled. The domains present in E. coli CheA are indicated; in addition, CheA contains a CheY-binding (P2) domain absent from ChpA. B, protein construct designations and corresponding domain architectures used in this study. The presence of conserved His, Asp, Thr, or Ser or its alanine (A) substitution is indicated.