TABLE 1.

Asn³⁴⁷ glycosylation, in particular the triantennary glycoforms, causes more perturbation of the average RCL conformation relative to the non-glycosylated RCL structure

Perturbation, which may restrict access of or affect recognition by the RCL-targeting proteases, was measured as the average root mean square deviation (Å) (mean ± S.D.) of the Cα atoms of the RCL over the course of the simulations (t = 100 ns, n = 1,000 sampled time points) of the Asn³⁴⁷ glycoforms of CBG relative to the average structure adopted by the RCL Cα atoms in the non-glycosylated CBG variant.

CBG Asn347 glycoform	Perturbation of the RCL conformation (RMSD) (mean ± S.D.)
	Å
Non-glycosylated	1.5 ± 0.4a
BS2	2.4 ± 0.3
TS3-A	4.1 ± 0.5
TS3-B	3.1 ± 0.4
TS3f-A	3.1 ± 0.4
TS3f-B	3.5 ± 0.7
DTS3-A	3.9 ± 0.4
DTS3-B	3.5 ± 0.6

^a This value represents the baseline deviation from the average RCL structure without perturbation by an attached glycan. See the legend to Fig. 1 and supplemental Table S1 for the Asn³⁴⁷ glycoform nomenclature used here.