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. 2016 Jul 5;291(34):17754–17771. doi: 10.1074/jbc.M116.727107

FIGURE 11.

FIGURE 11.

Effect of native LdTyrRS on the cytokine secretion profile of mouse macrophages. A and B, secretion profile of TNF-α (A) and IL-6 (B) using the L. donovani promastigote culture supernatants alone (Cult. Sup.), promastigote culture supernatants neutralized with the anti-TyrRS antibody (Sup + α-TyrRS Ab), native LdTyrRS immunoprecipitated from promastigote culture supernatant (PD-TyrRS), and finally native LdTyrRS (PD-TyrRS) neutralized with its own antibody (PD-TyrRS + α-TyrRS Ab). Recombinant LdTyrRS was used as a positive control. TNF-α and IL-6 secretion are clearly enhanced significantly when immunoprecipitated native LdTyrRS (PD-TyrRS) is used. The triggering activity of native LdTyrRS (PD-TyrRS + α-TyrRS Ab) is reduced when preincubated with anti-LdTyrRS antibodies (1:3000), indicating specific activation of macrophages with native LdTyrRS (PD-TyrRS). The results represent mean ± S.D. with n = 3. Tukey's test was performed, and p values are indicated.