Subcellular localization of LdTyrRS in L. donovani. Immunofluorescence analysis by confocal micrograph of wild-type log phase promastigotes. Panel A, phase-contrast image. Panel B, promastigotes stained with DAPI. Panel C, anti-LdTyrRS antibody detected using Alexa 542 (red)-conjugated secondary antibody. Panels D and E, merged micrographs. k and n indicate kinetoplastid and nuclear DNA, respectively. The scale bar represents 10 μm.