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. 2016 Jun 23;291(34):17848–17860. doi: 10.1074/jbc.M115.712398

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — Single channel recording from planar bilayers containing reconstituted OEP40. A, single channel recordings in symmetrical buffer conditions (250/250 mm KCl) from bilayers containing a single active OEP40 channel in response to an applied voltage gate of the indicated voltage amplitude (V_m). B, mean variance histogram of the current recording at V_m = 100 mV (top) and V_m = −100 mV (bottom). C, mean variance plot of the current recording at V_m = 100 mV (top) and V_m = −100 mV (bottom). D, current-voltage ramp (−100 to 100 mV, sweep-rate 15 mV/s) of a single open OEP40 channel in symmetrical buffer (see A). E, current-voltage ramp (−80 to 80 mV, sweep-rate 15 mV/s) of a single open OEP40 channel in asymmetrical buffer (250/20 mm KCl). F, voltage-dependent open probability of OEP40 single channels (n = 3).