Skip to main content
. 2016 Jun 23;291(34):17848–17860. doi: 10.1074/jbc.M115.712398

FIGURE 5.

FIGURE 5.

Effect of Tre-6-P on single channel activity of reconstituted OEP40. A, current recording from a bilayer containing a single active OEP40 channel in response to an applied voltage gate of Vm = −60 mV in the absence (top) and presence of 1 mm Tre-6-P (cis/trans). Symmetrical buffer conditions (250/250 mm KCl). B, voltage-dependent open probabilities (n = 3) of the OEP40 channel in the absence (black square) and presence of 1 mm Tre-6-P (red circle) (cis/trans). C, apparent conductance Gmax obtained from the maximal current amplitudes (Imax) at the given voltages in the absence (black square) and presence of 1 mm Tre-6-P (red circle) (n = 3). D, current-voltage ramp (−80 to 80 mV sweep-rate 15 mV/s) of a single open OEP40 channel in symmetrical buffer (20/20 mm KCl) in the presence of 1 mm Tre-6-P in the cis compartment. E, current-voltage ramps (−80 to 80 mV sweep-rate 15 mV/s) of a single open OEP40 channel in asymmetrical buffer (250/20 mm KCl) in the absence (upper panel) and presence of 1 mm Tre-6-P in cis (lower panel). F, same as E except the asymmetric 20/100 (cis/trans) KCl buffer conditions.