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. 2016 Jun 24;291(34):17861–17880. doi: 10.1074/jbc.M116.718536

FIGURE 9.

FIGURE 9.

SOCS2-AS1 knockdown sensitizes cells to docetaxel treatment and increases apoptosis. A, knockdown efficiency of SOCS2-AS1 by siRNA as analyzed by qRT-PCR (n = 3) in LNCaP and LTAD cells treated with docetaxel for 7 days. B, MTS assay of LNCaP and LTAD cells transfected with 20 nm siSOCS2-AS1, siSOCS2, or siNC for 24 h following 1 nm docetaxel or DMSO treatment for the indicated times. C and D, TUNEL assay in LNCaP and LTAD cells transfected with 20 nm siSOCS2-AS1, siSOCS2, or siNC for 24 h following 1 or 2 nm docetaxel treatment for 24 h. C, representation of DAPI (upper panels) and Alexa Fluor 488 (lower panels) signals detected in LNCaP cells treated with docetaxel. D, graph representing the quantification of apoptotic cells in LNCaP and LTAD cells. E, TUNEL assay in VCaP cells. VCaP cells transfected with 20 nm siSOCS2-AS1, siSOCS2, or siNC for 24 h following 2 nm docetaxel treatment for 24 h. F, MTS assay of VCaP cells transfected with 20 nm siSOCS2-AS1, siSOCS2, or siNC for 24 h following 2 nm docetaxel or DMSO treatment for the indicated times. Data represent the average of three different views (n = 3). Values represent mean ± S.D. *, p < 0.05; **, p < 0.01 (versus siNC).