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. 2016 Jun 30;291(34):17953–17963. doi: 10.1074/jbc.M116.724401

FIGURE 2.

FIGURE 2.

N terminus of AIRE binds to FBXO3. A, schematic representation of WT and mutant AIRE proteins. Black lines below the schematic indicate FLAG epitope-tagged deletion mutant AIRE peptides. B, N terminus of AIRE interacts with FBXO3. FLAG epitope-tagged WT and mutant AIRE proteins (f:AIRE) were co-expressed with the V5 epitope-tagged FBXO3 (v:FBXO3) protein in 293T cells. Anti-FLAG immunoprecipitations were performed. The lower panels depict total lysates (lanes 1–5, 3%) and immunoprecipitated (lanes 6–10) WT and mutant AIRE proteins. The upper panels depict the V5 epitope-tagged FBXO3 protein in lysates (lanes 1–5) and co-immunoprecipitations (lanes 6–10) with WT and mutant FLAG epitope-tagged AIRE proteins.