FIGURE 3.

Effects of TGF-β1 on expression of Jun family members in DU145 and PC3 cells. A, levels of mRNA of c-Jun, JunB, and JunD in DU145 and PC3 cell after treatment with TGF-β1 (5 ng/ml) as determined by RT-PCR. B, the protein levels of c-Jun, JunB, and JunD from whole cell lysates of DU145 and PC3 cells after treatment with TGF-β1 (5 ng/ml) at different time points as determined by Western blotting analysis. C, quantitative analysis of relative levels of c-Jun, JunB, and JunD proteins in DU145 and PC3 cells after treatment with TGF-β1. Normalization was performed relative to the signal obtained with β-actin. Each bar represents mean ± S.E. (error bars) (n = 3). *, significantly different from untreated controls (p < 0.05).