FIGURE 4.

Role of individual Jun family members in proliferation of DU145 and PC3 cells. DU145 and PC3 cells were transfected with either control or specific Jun protein siRNA to knock down expression of individual family members. A, cell proliferation in DU145 and PC3 cells after transfection with control (siControl-A), or JunB, JunD, or c-Jun siRNA. Each bar represents mean ± S.E. (error bars) (n = 3). *, significantly different when compared with appropriate controls (p < 0.05). Levels of Jun proteins after transfection with control siRNA and specific Jun siRNAs were determined by Western blotting analysis (inset). B, cell proliferation in DU145 and PC3 cells after transfection with control or JunD siRNA and treatment with TGF-β1 (5 ng/ml) for 4 days.