. 2016 Jun 28;291(34):18016–18029. doi: 10.1074/jbc.M116.732032

TABLE 1.

Identification of IgE antibody-binding peptides of Rhi o 1 allergen

Critical residues selected for mutagenesis are also listed. IgE binders are shown with asterisks. Prediction score, confidence score of ABCpred server (threshold >0.5); A_pos., A₄₀₅ value of positive control (intact rRhi o1); A_pep., A₄₀₅ value of individual peptides (P1–P5); A_neg., A₄₀₅ value of negative controls (HSA and random peptide); ELISA results (A₄₀₅ values of bound IgEs) are the means of triplicate determinations with variations <5%.

Peptide no.	Sequence of putative B-cell epitopes predicted by the servers	Prediction score	IgE binding (ELISA) data		Mutations in critical residues
Peptide no.		Prediction score	A_pos./A_pep.	A_pep./A_neg.	Mutations in critical residues
*P1	⁴⁴`TGEYLTQKYFNSQRNN`	0.96	2.3	16.0	T49A/Y52A
P2	⁹⁰`EIEIGTPPQPFTVVFD`	0.95	22.0	1.8
P3	²⁵²`EGDIHWSDVRRKGYWE`	0.95	25.5	1.7
P4	²⁴⁴`GGVDEDHFEGDIHWSD`	0.89	29	1.4
*P5	³¹¹`GAEKNWAGQYVVDCNK`	0.87	1.9	19.8	K314A/W316A