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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Biol Psychiatry. 2016 Mar 8;80(11):878–887. doi: 10.1016/j.biopsych.2016.02.031

Figure 1.

Figure 1

NMUR2 is expressed synaptically in the NAcSh. (a) Brain slice illustration (left) showing the site in the NAcSh where confocal microscopy images (63×) of NMUR2 immunofluorescence (right) were taken. Scale bar 5 µm. (b) Representative images of electron microscopy in the NAcSh with NMUR2 immunogold staining highlighted by blue arrows. Synapses can be seen as the dark lines of electron density. Scale bar 100 nm. (c) Summary of the synaptic localization of NMUR2 from 166 electron microscopy images. Proximity of a gold piece to a synapse was defined as follows: synaptic is < 50 nm from synapse, perisynaptic is 50 to 150 nm from synapse, and nonsynaptic is > 150 nm. Presynaptic and postsynaptic localization was determined by identification of clear vesicles and the postsynaptic density, respectively.