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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Nat Struct Mol Biol. 2016 Aug 1;23(9):821–829. doi: 10.1038/nsmb.3272

Figure 1. Structural context of approaches to test Hsc70 disassembly mechanisms.

Figure 1

A: Cut-away view of clathrin cage (interior surface in cyan; exterior in yellow/orange) with auxilin (magenta; pdb 1XI523). C-termini of CHCs form a helical tripod (red) under each vertex. B: Expanded view of boxed region from A. Hsc70 (pdb 4B9Q3; blue) modeled into the clathrin:auxilin cage based on an Hsp70 NBD:auxilin J domain structure26 positions its protein binding domain (PBD) to bind the terminal tail (red circles with Hsc70 binding QLMLT sequence in green). C: Sequences of the termini of the CHCs used (#1 is WT), with helical segments in red, and Hsc70 binding and FLAG sites in magenta.