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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Nat Struct Mol Biol. 2016 Aug 1;23(9):821–829. doi: 10.1038/nsmb.3272

Figure 5. Scattering increases due to Hsc70 binding reflect multiple Hsc70s binding per CHC, not cage expansion.

Figure 5

A. SDS PAGE of pellets of the indicated cages incubated with 1, 3, or 9 μM Hsc70 (lanes 1–9) or Hsc70ΔC (lanes 10–18; [Hsc70] and [Hsc70ΔC] increase from left to right as indicated). Lanes 19–24 show experiments without cages. B: As in A, but using FLAG cages and Fab (lanes 10–12 are no cage control). C: Hsc70/CHC or Hsc70ΔC/CHC ratios plotted vs. [Hsc70] or [Hsc70ΔC], as indicated. D: As in C, but using FLAG cages and Fab.