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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: J Occup Environ Hyg. 2016 Nov;13(11):829–839. doi: 10.1080/15459624.2016.1183013

FIGURE 1. Immunoreactivity of MDI-HSA Conjugates.

FIGURE 1

(A) MDI-HSA ELISA and (B) MDI-MDI ELISA analyses of the immunoreactivity of conjugates prepared using different methods. For (A) and (B) IgM anti-MDI mAb 15D4 was used as a capture antibody. Conjugates prepared using either the fast, drip, infusion, or 2-phase methods were used as antigen and either an anti-HSA antibody (A) or anti-MDI IgG mAb DA5 (B) were used as the detection antibody. Results show the average of three different conjugates prepared the same way. ELISAs are corrected using unconjugated HSA as a control. For (B) fast method ELISA absorbances were significantly less than that from drip, infusion or 2-phase methods. (C) Correlation between results from (A) on the y-axis and (B) on the x-axis. * denotes p<.05; ** denotes p<.0001.