Figure 4. Immunoreactivity of Conjugates.
Five individual conjugates from each group were chosen based on results from Figure 1 B. (A) Immunoreactivity of conjugates in a sandwich ELISA with IgM mAb 15D4 as a capture and IgG1 mAb DA5 as a detection antibody. (B) Immunoreactivity of conjugates coated directly on the plate and detected using mAb DA5. (C & D) Antigenicity of the conjugates with human serum IgG (C) or IgE (D) from a representative sensitized individual. All assays are corrected using unconjugated HSA as a control.