Figure 3.

Agonist-mediated internalization of receptors. Cells expressing (A) B₂R, (B) MasR or (C and D) B₂R-MasR were incubated in the absence (control, black bar) or presence of (1 µmol/L) BK (white bar) or (1 µmol/L) Ang-(1–7) (patterned bar) for 15 min, and the percentage of B₂R (A and C) or MasR (B and D) present in the membrane was analyzed by ligand binding assay as described under Methods. * P < 0.05 vs. control. (E) Effect of B₂R coexpression on the time course of MasR sequestration. Cells expressing MasR (solid line) or B₂R-MasR (dotted line) were incubated with Ang-(1–7) (1 µmol/L) for the indicated time intervals and receptor sequestration determined as described under Methods. Values are mean+SEM of four different experiments each performed in triplicate.