Fig. 9.
Influence of disrupted CHO cells on amplification efficiency for a mycoplasmal target sequence. Real time PCR was performed using 5 ng (1.54 × 109 copies) of plasmid encoding a mycoplasmal DNA sequence and a further 8 tenfold dilutions of the plasmid template. For each starting solution either zero cells were present or disrupted cells derived from samples of 2 × 106 cells/mL, from shake flask cultivation, or 2.5 × 105, 6 × 106 and 1 × 107 cells/mL, from bioreactor cultivation, were added to plasmid DNA (see legend). The resultant Cq values for each amplification reaction were plotted as a function of sample dilution. Data featured is typical of N = 3 analytical repeats