Fig. 1.

Transcriptomic profiling of pancreatic alpha, beta and delta cells. RNA was extracted from purified populations of alpha, beta and delta cells, and converted to cDNA or prepped for RNA sequencing. (a) Populations of alpha (black bars), beta (grey bars) and delta (white bars) cells were checked for Ins, Gcg and Sst enrichment, respectively, using qPCR analysis. Data are presented as the geometric mean, with error bars (SEM) calculated from log₂ data. Each column represents the average expression from three separate samples. Significance comparisons were calculated by one-way ANOVA with Bonferroni post hoc comparison; ***p < 0.001. (b) RNA from five alpha cell samples, four beta cell samples and six delta cell samples was sequenced using SE50 sequencing. Differential gene expression was determined using edgeR, and a principle component analysis plot was constructed using a false discovery rate of 5% and a sensitivity threshold of FPKM values >1. (c) Pie chart showing the cellular distribution of genes differentially expressed between islet cell types. (d) Pie chart showing the distribution of differentially expressed genes found at the plasma membrane. (e) Heatmap showing the top 40 most differentially expressed genes found at the plasma membrane. Data are presented as log₂ FPKM