FIGURE 6.
Visualization of prepores of 8-69OX FraC with AFM. a, two-dimensional packing of ring-shaped oligomers of 8-69OX FraC on supported lipid bilayers composed of the lipid mixture SM/DOPC (1:1). b, diameter distribution analysis (peak-to-peak distances of the protein protrusion in the height profile). The average diameter was 62 ± 7 Å (mean ± S.D. from the Gaussian distributions). Inset, detail of the particles inside the white dashed rectangle in a. The slightly smaller diameter compared with the WT suggests a tighter association of the subunits in the 8-69OX FraC mutant. c, magnification (12-nm size frame) of a single prepore of FraC 8-69OX (white square in a). d, cross-section profile (left to right) of prepore particles of FraC 8-69OX (white line in a). The molecules packed with a center-to-center distance of ∼108 Å.